Volume 10, Issue 4 (2-1997)
Med J Islam Repub Iran 1997 |
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From the Institute of Biochemistry and Biophysics, University of Tehran, P.O. Box 13145-1384, Tehran, Islamic Republic of Iran.
Abstract: (4009 Views)
In this study the programmed cell death (apoptosis) of human neutrophilic
granulocytes was investigated in the presence and absence of fetal calf serum (FCS),
cycloheximide and actinomycin-D. The results show that when FCS is omitted from
cultures, apart from a decrease in viability, the percentage of apoptotic cells and DNA
fragmentation increases. Apoptosis is accelerated in serum withdrawal cultures at 6
hours of incubation time. The use of fluorescent dyes and diphenylamine reaction
proceaures confirm the above results. Treatment of cells with protein synthesis
inhibitors, actinomycin-D and cycloheximide promotes apoptosis and produces a
typical ladder of internucleosomal cleavage in the cellular chromatin the extent of
fragmentation however, differs.
Type of Study: Original Research: Basic Science in Medicine |
Subject:
Biological Sciences
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