FAYAZ F, MOASAMI N, ZAIEM M, MOTABAR M, HOLAKOUEI NAEINY K. LOCAL PRODUCTION OF PRIMARY POWDER OF BACILLUS THURINGIENSIS SEROTYPE H-14 IN IRAN AND DETERMINATION OF ITS INSECTICIDAL PROPERTIES AGAINST CULEX PIPIENS AND ANOPHELES STEPHENSI. Med J Islam Repub Iran 1988; 2 (3) :229-236
URL:
http://mjiri.iums.ac.ir/article-1-1254-en.html
Iranian Research Organization For Science and Technology
Abstract: (4601 Views)
Three media formulated from molasses and cornsteep liquor and
another media based on the basal medium formulation of IPS-82 were
assessed for the growth and production of insecticidal properties of B. thuringiensis
H-14 .• Bacterial powders prepared from the broth cultures were
assayed against the larvae of Culex pipiens and Anopheles stephensi. A
standard powder of IPS-82 was included in the assay for comparison. Good
. growth was obtained in all the media and all powders were effective against
the two types of mosquito larvae. Media with the basal medium formulation
of Institute Pasteur Paris were the most effective. Molasses III media was
also very favorable. The concentrations required to kill 50% of the larvae of
Culex pipiens and Anopheles stephensi for these two were as follows:
- Media with the basal medium formulation of Institute Pasteur Paris:
24hr. Lc50 and it's 95% confidence limits of Culex pipiens and Anopheles
stephensi = 0.0025,0.0031,0.0018 mg/lit
0.1792,0.2603,0.1508 mg/lit
- Molasses III Media:
24hr. Lc50 and it's 95% confidence limits of Culex pipiens and Anopheles
stephensi = 0.0103,0.0115,0.0092 mg/lit
0.1994,0.2223,0.1769 mg/lit
The investigation shows that these media can be used for the production
of B.thuringiensis H-14 primary powder.