Volume 5, Issue 1 And (9-1991)                   Med J Islam Repub Iran 1991 | Back to browse issues page

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GOLIAEI B, DEIZADJI A, RABBANI A. HETEROGENEITY OF MACROPHAGE POPULATIONS: ANTIBODIES DETECTING VARIOUS POPULATIONS OF MACROPHAGES. Med J Islam Repub Iran 1991; 5 (1) :49-54
URL: http://mjiri.iums.ac.ir/article-1-1523-en.html
HETEROGENEITY OF MACROPHAGE POPULATIONS: ANTIBODIES DETECTING VARIOUS POPULATIONS OF MACROPHAGES
B GOLIAEI , A DEIZADJI , A RABBANI
From tile Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Islamic Republic of Iran.
Abstract:   (3951 Views)
Two groups of antibodies against mouse resident alveolar and peritoneal macrophages have been raised individually in rabbits by immunization with whole cell suspensions of lavaged alveolar and peritoneal macrophages. The whole antisera reacted positively with neutrophils and lymphocytes. However, upon extensive absorptions with these cells the activity became restricted to macrophages. The immunoglobulins were purified up to 8 fold by repeated precipitations with saturated ammonium sulphate. The antibodies were labeled with fluorescein isothiocyanate and further used. The specificity of antibodies was confirmed by direct and indirect immunofluorescence and complement-dependent cytotoxicity. In the cytolysis of target cells in the presence of complement, the antibodies could lyse more than 80% of cells at dilutions as low as 1:512.
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Type of Study: Original Research: Basic Science in Medicine | Subject: Biological Sciences
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