Medical Journal of The Islamic Republic of Iran (MJIRI)
Iran University of Medical Sciences
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Volume 16, Issue 3 (11-2002)
Med J Islam Repub Iran 2002 |
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AHMADI S, KARIMIAN S, SOTOUDEH M, BAHADORI M. HISTOLOGICAL AND IMMUNOHISTOCHEMICAL STUDY OF PANCREATIC ISLET BETA CELLS OF DIABETIC RATS TREATED WITH ORAL VANADYL SULPHATE. Med J Islam Repub Iran 2002; 16 (3) :173-178
URL: http://mjiri.iums.ac.ir/article-1-750-en.html
URL: http://mjiri.iums.ac.ir/article-1-750-en.html
From the Departments of Physiology School of Medicine,Tehran University of Medical Sciences, Tehran, I.R. Iran.
Abstract: (5304 Views)
Vanadium salts have been suggested as a possible therapeutic agent for the
treatment of diabetes. The aim of the present study was to clarify histological and
immunohistochemical changes that occur in the pancreatic beta (β) cells of vanadyl
sulphate (VS)-treated streptozotocin (STZ) induced diabetic rats.
Male Wi star rats were made diabetic by injecting a single intravenous dose
of STZ (40 mg/kg) and were divided into two groups seven days after STZ injection.
In the first group VS was administered via drinking water at a concentration
of 1 mg/mL and treatment was maintained until normoglycemia appeared (DT).
A second group of diabetic animals received distilled water for the same period
and were considered as control diabetic (DC). One group of animals (NC) was
injected intravenously with the same amount of vehicle as the diabetic rats and
was considered as non-diabetic control. VS treatment was accompanied by amelioration
of the signs of diabetes in DT rats while DC animals remained diabetic
during this period.
Hemotoxylin - Eosin stained pancreatic sections of DC rats showed a decrease
in the number and size of islets and a disruption in their architecture. In DT
rats the histological appearance of the islets was normal, their shape and size
being within normal limits.
In horseradish peroxidase procedure (using guinea pig antiserum to insulin
as primary antibody) performed on pancreatic islet paraffin sections of rats, insulin
immunoreactivity was found in the majority of the islets in DT rats while in
the islets of DC rats immunoreactivity was rare.
The results of this study indicated that amelioration of diabetes in vanadyl
sulphate treated diabetic rats was accompanied with well preservation of islet
structure and insulin immunoreactivity.
Type of Study: Original Research: Basic Science in Medicine |
Subject:
Physiology
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