Volume 13, Issue 1 (5-1999)                   Med J Islam Repub Iran 1999 | Back to browse issues page

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FARHOUDI-MOGHADDAM A A, DOMANSKY N N, ZIAEE A. EXPRESSION OF HEPATITIS B SURFACE ANTIGEN IN SACCHAROMYCES CEREVISIAE. Med J Islam Repub Iran 1999; 13 (1) :67-73
URL: http://mjiri.iums.ac.ir/article-1-975-en.html
EXPRESSION OF HEPATITIS B SURFACE ANTIGEN IN SACCHAROMYCES CEREVISIAE
ALI A FARHOUDI-MOGHADDAM , NICKOLAY N DOMANSKY , A.A ZIAEE
From the Molecular Biology Unit . Pasteur Institute. Tehran. I.R. Iran
Abstract:   (4206 Views)
The genome of HB V virus of serotype ayw cloned in pBR322 and expression shuttle vector p YES2 were used for construction of the HBsAg chimeric genes and their expression in Saccharomyces cerevisiae. Two recombinant plasmids were constructed. One of them contained the coding sequences for the major polypeptide of surface antigen. Another construct carried the major polypeptide with the pre-S2 antigenic determinant. These vectors were transferred into the yeast. Only pDFl which contained the HBsAg gene was expressed. Some peculiar features of recombinant plasmid construction and expression of the HBsAg gene are discussed
Keywords: HBV surface antigen, recombinant plasmids, Saccharomyces cerevisiae exoression vector.
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Type of Study: Original Research: Basic Science in Medicine | Subject: Biological Sciences
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