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APPLICATION AND ADAPTATION OF DOT IMMUNOASSAY TO DETECT AND MEASURE RUBELLA VIRUS ANTIBODY
T Bamdad, Mh Rodstai, H Solimandjahi, M Malekaneh,
Volume 15, Issue 3 (11-2001)
Abstract
A dot immunobinding assay ( DIA) was used for a quantitative and qualitative assay of rubella antibody. Purified antigen and conjugated anti-human immunoglobulin (RAHlg) were prepared. Nitrocellulose paper dotted with the antigen was added to serially diluted sera or blood samples. The reacting antibodies were visualized by a peroxidase system. Development of a colored insoluble substrate was taken as a positive result. The adapted DIA was applied to test 105 serum samples. The sensitivity and specificity and immune titer of DIA were compared with hemagglutination inhibition (HI) test.
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