Showing 5 results for MOSAVI
Salah Rahmani, Mehdi Forozandeh, Mirlatif Mosavi, Abbas Rezaee,
Volume 19, Issue 4 (2-2006)
Abstract
Background: There is a conserved portion in the 16S rRNA gene of bacteria
which can be amplified by the universal PCR method. This fragment is 996 bp in length.
In this method, only one set of universal primers is used for the amplification of the
conserved region of the 16S rRNA gene, in common bacterial pathogens. Therefore,
using the universal PCR method, these bacteria are detectable only by one set of primers
then for detection of the bacteria, the PCR products are digested by the restriction
endonucleases. Since the restriction patterns of bacteria (RFLP) are expected to be
different from each other, on that basis we can identify the bacteria.
Methods: The conserved fragments of the 16S rRNA genes ofthe following
bacteria were amplified by the universal PCR method: Streptococcus pyogenes, Escherichia
coli, Pseudomonas aeruginosa and Neisseria gonorrhoeae. The PCR
products were digested by BsuRI (Hae III) restriction endonucleases and were electrophoresed
on agarose gel.
Results: The restriction patterns of these bacteria were different. Thirty isolated
E. coli and 28 isolated S. pyogenes from clinical samples were studied by this method.
The size of PCR products and RFLP patterns of every bacterium were the same as
standard strains. In comparison with culture method, the sensitivity of the universal
PCR is 92.3 %.The sensitivity of this method was determined up to about 11 and 190
bacteria for gram negatives and gram positives respectively.
Conclusion: These studies suggest that the universal PCR method accompanied
with RFLP is a very useful and rapid method, for detection and identification of bacteria
in body fluids.
Seyed Reza Kazemi Nezhad, Fatemeh Mosavi, Ali Akbar Momen, Hamid Galehdari, Gholamreza Mohamadian,
Volume 25, Issue 4 (12-2011)
Abstract
Background: Spinal muscular atrophy (SMA) is the second most common lethal autosomal recessive disease. It is a neuromuscular disorder caused by degenerative of lower motor neurons and occasionally bulbar neurons leading to progressive limb paralysis and muscular atrophy. The SMN1 gene is recognized as a SMA causing gene while NAIP has been characterized as a modifying factor for the clinical severity and age at disease onset in SMA patients (SMA subtypes). The relationship between NAIP deletion and type of SMA remains to be clarified we investigated this gene alteration in all types of SMA patients.
Methods: Molecular analysis was performed on fifty patients with a clinical diagnosis of SMA in Khuzestan province. In addition to common PCR-RFLP analysis for exon 7 and 8 of SMN1 gene, as an internal control we analysed NAIP deletion with PCR of exon 5 of this gene in a multiplex PCR with exon 13 of it.
Results: Homozygous-deletion frequency rate for the telomeric copy of SMN (SMN1) exon 7 in all types (type I, II, Ш) of SMA was approximately 90% and the frequency of deletion in exon 7 and 8 together in all types estimated about 70%. Moreover NAIP gene was deleted in about 60% of these patients and this shows deletion in 91% of type I SMA patients. The correlation between NAIP-deletion and SMN1 mutation showed a high frequency rate.
Conclusion: In this study, high frequency of NAIP gene deletion in all type of disease shows the importance role of it in disease pathogenesis. High frequency of NAIP deletion in SMA type I, also shows the importance of the gene in type and severity of disease so it may be a modifier factor in severity of disease.
Kobra Z Entezami, Tahere Mosavi,
Volume 31, Issue 1 (1-2017)
Abstract
Background: Thermal burn injuries impair the host defence system. Hence, in the present study, we aimed at investigating the changes in the number and phenotype of peripheral blood lymphocyte populations (T, B, and natural killer cells) and their subpopulations in patients with thermal burns and determining the relationships with different sizes of total body surface area (TBSA).
Methods: Blood samples from 67 patients, admitted to Motahary Burn Center in Tehran, with burns from 30% to more than 70% TBSA were collected on Days 3 and 7 postburn. Lymphocytes and their subpopulations were identified by monoclonal antibodies. The cells were analyzed using flow cytometry. The results were compared with healthy controls.
Results: In this study, 3 and 7 days after burn injury, the percentages of CD3+, CD4+ and CD8+ lymphocyte significantly decreased, CD4+/CD8+ ratios were below the normal range, and CD19+ (B cells) significantly increased. No significant difference was obtained in the mean percentage of CD16+ (NK cells) between Days 3 and 7 postburn. Patients with burns of 30% TBSA or greater (>70%) had a significant reduction in CD3+, CD4+ and CD8+ ( T cells) numbers up to 7 days compared with 3 days after burn injury. Patients with 30% to >70 % TBSA burn failed to show any significant changes in CD4+/CD8+ ratio as well as CD16+ (NK cells) 3 to 7 days after burn. In patients with burns more than 30% to>70% TBSA, CD19+ (B cells) number changes were found to be complicated after 3 and 7 days.
Conclusion: The results of this study suggest that alterations of immune cell surface markers and TBSA% can reflect postburn lymphocyte activation.
Kamyar Mansori, Masoud Solaymani-Dodaran, Alireza Mosavi-Jarrahi, Ali Ganbary Motlagh, Masoud Salehi, Alireza Delavari, Ali Hosseini, Mohsen Asadi-Lari,
Volume 33, Issue 1 (2-2019)
Abstract
Background: This study aimed to determine effective factors on geographic distribution of the Incidence of Colorectal Cancer (CRC) in Tehran, Iran using Geographically Weighted Poisson Regression Model.
Methods: This ecological study was carried out at neighborhood level of Tehran in 2017-2018. Data for CRC incidence was extracted from the population-based cancer registry data of Iran. The socioeconomic variables, risk factors and health costs were extracted from the Urban HEART Study in Tehran. Geographically weighted Poisson regression model was used for determination of the association between these variables with CRC incidence. GWR 4, Stata 14 and ArcGIS 10.3 software systems were used for statistical analysis.
Results: The total number of incident CRC cases were 2815 in Tehran from 2008 to 2011, of whom, 2491 cases were successfully geocoded to the neighborhood. The median IRR for local variables were : unemployed people over 15 year old (median IRR: 1.17), women aged 17 years or older with university education (median IRR: 1.17), women head of household (median IRR: 1.06), people without insurance coverage (median IRR: 1.10), households without daily consumption of milk (median IRR: 0.85), smoking households (median IRR: 1.07), household’s health expenditure (median IRR: 1.39), disease diagnosis costs (median IRR: 1.03), medicines costs of households (median IRR: 1.05), cost of the hospital (median IRR: 1.09), cost of medical visits (median IRR: 1.27).
Conclusion: The spatial variability was observed for most socioeconomic variables, risk factors and health costs that had effects on CRC incidence in Tehran. Spatial variability is necessary when interpreting the results and utterly helpful for implementation of prevention programs.
Mohsen Akhavan Sepahi, Majid Mosavimovahed,
Volume 35, Issue 1 (1-2021)
Abstract
The occurrence rate of nephrolithiasis has increased steadily. The development of stone in children depends on certain physicochemical factors. Kidney stones are one of the challenges of pediatric nephrology. We brief reviewed the current literature on the best diagnostic imaging to decrease the complication of nephrolithiasis, and we considered the areas that need future research.
