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Showing 3 results for STANFORD
VACCINES CONTAINING MYCOBACTERIUM VACCAE AND THEIR USE IN THE CHILDREN OF LEPROSY PATIENTS IN IRAN
Kiumars Ghazi Saidi, John L. Stanford , Cynthia A. Stanford, Yahya Dowlati,
Volume 8, Issue 2 (8-1994)
Abstract
The policy of vaccinating children who live with leprosy patients, and who have responses to leprosin A of2rnrn or less, with BCG+killed Mycobacterium vaccae if they lack a BCG scar, or with killed M. vaccae alone if they have a BCG scar, has been followed over 3-4 years in two centers in Iran. Judged on the basis of skin test conversion to leprosin positivity, the policy has been highly successful. A way in which the vaccines may work is discussed, and supported by differences in apparent efficacy between the two study centers.
A RAPID AND SIMPLE METHOD OF DNA EXTRACTION FROM CLINICAL SPECIMENS CONTAINING MYCOBACTERIUM LEPRAE FOR PCR ASSAYS
Abdolnasser Rapi, Mohammed H. Mobayen, Francois Feval, John L. Stanford,
Volume 10, Issue 3 (11-1996)
Abstract
We describe a rapid, simple, and reliable procedure for routine isolation of Mycobacterium leprae DNA from slit-skin swab specimens. This one-step DNA extraction method is based on the utilization of ChelexR 100, a chelating ion exchange resin. Slit-skin swab specimens from seven untreated leprosy patients at Baba Baghi Leprosy Hospital in Iran were processed by this procedure. The polymerase chain reaction (PCR) was performed with primers for a 530-base pair (bp) fragment of the gene encoding the 36 kDa antigen of M. leprae. All the specimens were found to be PCR -positive, suggesting the efficiency of the present DNA extraction method.
THE DETECTION OF Mycobacterium tuberculosis BY PCR IN SPUTUM SAMPLES FROM LONG-TERMTREATED LEPROSY PATIENTS: ASSOCIATIONS WITH SKIN TEST RESULTS AND IMMUNOTHERAPY WITH Mycobacterium vaccae
A Rafi, A Ahmadlan, Mh Mobayen, F Feval, Y Dowlati, K Ghazi Saidi, Aa Velayati, Jl Stanford,
Volume 11, Issue 1 (5-1997)
Abstract
As part of a series of investigations at Baba Baghi Leprosarium in lran, 44 long-treated leprosy patients were selected for our study. Samples of early morning sputum were obtained from each patient, examined by microscopy for acid-fast bacilli (AFB), and cultured for tubercle bacilli. These tests were negative, but the polymerase chain reaction (PCR) for an insertion sequence believed to be specific for Mycobacterium tuberculosis was applied to each sputum sample and those from six patients were found to be positive. Five of the six positive samples were from the 21 patients producing Koch-type responses to tuberculin, and none were from the 11 patients previously found to have skin-tissue fluid or sputum positive by PCR for Mycobacterium leprae. Whereas immunotherapy with killed Mycobacterium vaccae given nearly 2 years earlier to 23 of the patients strongly influenced PCR results for M. leprae (p= 0.0 I), it had no influence on results fm' tubercle bacilli. However, at a second sampling date 18 months later, the only 2 patients still positive by PCR for tubercle bacilli came from the placebo recipient group. The possible significance of the findings is discussed.
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