Showing 3 results for Alkaline Phosphatase
Aa Pourfathollah, Mb Eslami, Ar Yalda, Af Sarraf Nejat,
Volume 10, Issue 3 (11-1996)
Abstract
Human brucellosis is a major health problem in developing countries. A
number of patients with human brucellosis do not recover from the acute stage of
the disease and pass into the chronic form. Since the pathophysiology of this
change is not well understood, we studied the T-cell subsets during the acute,
subacute and chronic forms of human brucellosis. In this study we found
alterations in T-Iymphocyte subsets and in the CD4/CD8 ratio in subacute and
chronic brucellosis while no significant change was found in T-cell subsets in
acute brucellosis. We believe that this alteration of T-cell subsets is important in
the pathophysiology of brucellosis, but it is not clear whether brucella cause this
change or a primary immunodysfunction in patients with a disturbance in T-cell
subset regulation prevents recovery from acute brucellosis and leads to the
development of chronic brucellosis.
A.a.n.p.m Dabare, A.m.e. Nouri, Mrc Path, R.t.d. Oliver,
Volume 14, Issue 1 (5-2000)
Abstract
In this study cellular phosphatase activity, referred to as enzyme developed
color (EDC) assay, was used for an in vitro assessment of cell proliferation and
cytotoxicity. Optimum conditions were established and found to be 10 x103 cells /
well at 37°C and 60 min incubation (for developing color). Under the same conditions
a direct correlation between optical density (00) and cell number was observed.
Thus, the ODs for a cell line, Ish at 100, 50, 25 and 10 X 103 cells/well were
1.0S ±0.05, 0.59 ±0.02, 0.33 ±0.02, and 0.17 ±0.01 respectively (r=0.995,p<0.001).
When EDC was compared with another colorimetric assay, i.e. MTT, the results
showed a direct correlation with r=0.995, p<0.001 .
Repeating the experiment with live and fixed tumor cells showed similar results.
Thus, for the Ish line, the ODs at 50 and 10 X 103 cells/well for fixed and live cells
were 0.66±0.01, 0.18±0.01 and O.64±0.03 (p>0.05), 0.19±0.01 (p>0.05),
respectively. The assay was also shown to be suitable for the measurement of cell
cytotoxicity and compared well with the MTT assay.
These findings indicated that a simple, rapid and economical EDC assay could
be used to investigate various cellular functions. The main advantage of EDC is it's
suitability to use stored cells. This provides flexibility for testing samples stored over
a long period in order to limit inter-experimental variations
Maryam Saboute, Aco Mahmoudian, Nasrin Khalesi, Zahra Vahedi, Nastaran Khosravi, Leila Allahqoli,
Volume 36, Issue 1 (1-2022)
Abstract
Background: Hyperbilirubinemia is one of the most common neonatal disorders and one of the risk factors of neurological complications. So this study was conducted to evaluate the correlation between alkaline phosphatase (ALP) and pathological jaundice.
Methods: A case-control was performed on term neonates with and without pathological jaundice who were referred to the Hazrat-e-Ali Asghar Hospital in 2017. In both groups, cases (neonates with pathological jaundice, n=153) and control (neonates with and without pathological jaundice, n=153) levels of alkaline-phosphatase and serum total bilirubin were evaluated with biochemical tests. Moreover, other data were also recorded from their history and clinical examinations. In addition, the severity of jaundice, duration of hospitalization, type of required treatment, and probable complications after the treatment were considered in follow-up. Data were collected by checklist and entered to SPSS v.20. ALP level and its relationship with serum total bilirubin compared between two groups.
Results: Mean level of ALP was 411.3 ± 134.2 U/L in the case group and 338 ± 131.4 U/L in the control group. Serum total bilirubin level was 11.9 mg/dl in the case group and 6.2 mg/dl in the control group. ALP levels in the case group were significantly more than the control group (p=0.001). There was no correlation between ALP and serum total bilirubin level in neonates in the case group (p=0.532). There was no statistically significant relationship between alkaline phosphatase level and gender of neonates, but the relationship of ALP level with types of delivery was statistically significant (p=0.002). There was not a significant relationship between ALP level with hospitalization duration (p=0.371).
Conclusion: The result of this study showed that there is no correlation between ALP levels and pathological jaundice in patients, although this issue needs to be approved by the other studies.
