Volume 12, Issue 3 (11-1998)                   Med J Islam Repub Iran 1998 | Back to browse issues page

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TABATABAIE YAZDI M, KHALEGHPARAST S, NAYEBPOUR S. PURIFICATION AND PARTIAL CHARACTERIZATION O F PEROXIDASES FROM CULTIVATED RAPHANUS SATIVUS L. VAR. CICIL. Med J Islam Repub Iran 1998; 12 (3) :273-277
URL: http://mjiri.iums.ac.ir/article-1-1006-en.html
PURIFICATION AND PARTIAL CHARACTERIZATION O F PEROXIDASES FROM CULTIVATED RAPHANUS SATIVUS L. VAR. CICIL
M TABATABAIE YAZDI , S KHALEGHPARAST , SM NAYEBPOUR
From the Department of Biotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences,
Abstract:   (4693 Views)
Two peroxidases (EC. 1.11.1.7), POD I and POD II were purified from the roots of cultivatedRaphanus sativus L. Var. Cicil by one step ion-exchange chromatography after fractionation by acetone. The molecular weight of these enzymes were 43000 and 41000 Daltons and RZ 1.2 and 2.0 for POD I and POD II, respectively. Both enzymes consisted of a single polypeptide chain on SDS-PAGE. The maximum activity of POD I was observed at pH 4.6 and 30°C and for POD II, at pH 6.5 and 60°C. The Km value of POD I for H202 was 7.26 mM and for POD II, 2 mM toward o-dianisidin. Both isoenzymes were stable for 48 hours in temperatures up to 40°C and stable in pH 4-8 for 3 hours.
Keywords: Peroxidase, Horseradish, Purification, Characterization.
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Type of Study: Original Research: Basic Science in Medicine | Subject: Biological Sciences
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