Volume 12, Issue 2 (8-1998)                   Med J Islam Repub Iran 1998 | Back to browse issues page

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SAMADI A, PULITI A, GOOSSENS M. USE OF VECTORETTE AND SUBVECTORETTE PCR FOR THE ISOLATION OF TERMINAL SEQUENCES FROM Y EAST ARTIFICIAL CHROMOSOME (YAC) CLONES. Med J Islam Repub Iran 1998; 12 (2) :129-133
URL: http://mjiri.iums.ac.ir/article-1-1018-en.html
USE OF VECTORETTE AND SUBVECTORETTE PCR FOR THE ISOLATION OF TERMINAL SEQUENCES FROM Y EAST ARTIFICIAL CHROMOSOME (YAC) CLONES
ABBAS SAMADI , ALDAMARIA PULITI , MICHEL GOOSSENS
From the Dept. of Biochemistry, Kashan Medical University, Kashan, Islamic Republic of Iran
Abstract:   (3628 Views)
Development of yeast artificial chromosome (Y AC) vectors, molecular cloning of large segments of chromosomal DNA, and their propagation in yeast cells has become feasible. Overlapping Y AC provides a route to the development of physical maps of entire mammalian chromosomes. A rapid method was developed to isolate and sequence termini of Y AC inserts quickly. The Y AC clone is digested with a range of restriction enzymes, and ligated with a linker at its ends. The digested fragments were amplified using modified vector specific primers and a universal linker primer. PCR products were sequenced and the information used to drive new sets of primers for screening of Y AC libraries to obtain overlapping clones and construct existing Y AC contig.
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Type of Study: Original Research: Basic Science in Medicine | Subject: Biological Sciences
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