Volume 10, Issue 3 (11-1996)                   Med J Islam Repub Iran 1996 | Back to browse issues page

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RAPI A, MOBAYEN M H, FEVAL F, STANFORD J L. A RAPID AND SIMPLE METHOD OF DNA EXTRACTION FROM CLINICAL SPECIMENS CONTAINING MYCOBACTERIUM LEPRAE FOR PCR ASSAYS. Med J Islam Repub Iran 1996; 10 (3) :233-235
URL: http://mjiri.iums.ac.ir/article-1-1190-en.html
A RAPID AND SIMPLE METHOD OF DNA EXTRACTION FROM CLINICAL SPECIMENS CONTAINING MYCOBACTERIUM LEPRAE FOR PCR ASSAYS
ABDOLNASSER RAPI , MOHAMMED H. MOBAYEN , FRANCOIS FEVAL , JOHN L. STANFORD
From the Department of Medical Microbiology, UCL Medical School, University of London, U.K
Abstract:   (3980 Views)
We describe a rapid, simple, and reliable procedure for routine isolation of Mycobacterium leprae DNA from slit-skin swab specimens. This one-step DNA extraction method is based on the utilization of ChelexR 100, a chelating ion exchange resin. Slit-skin swab specimens from seven untreated leprosy patients at Baba Baghi Leprosy Hospital in Iran were processed by this procedure. The polymerase chain reaction (PCR) was performed with primers for a 530-base pair (bp) fragment of the gene encoding the 36 kDa antigen of M. leprae. All the specimens were found to be PCR -positive, suggesting the efficiency of the present DNA extraction method.
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Type of Study: Original Research: Basic Science in Medicine | Subject: Microbiology and Anatomy
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