Volume 2, Issue 2 (8-1988)                   Med J Islam Repub Iran 1988 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BRU-CAPDEVILLE A C, MOTTEIAN NAJAR H, HINRICH PETERS J. DIFFERENTIATION OF MONOCYTE DERIVED DENDRITIC CELLS IN SERUM FREE CONDITIONS. Med J Islam Repub Iran 1988; 2 (2) :91-98
URL: http://mjiri.iums.ac.ir/article-1-1259-en.html
DIFFERENTIATION OF MONOCYTE DERIVED DENDRITIC CELLS IN SERUM FREE CONDITIONS
ANNE CHRISTINE BRU-CAPDEVILLE , HOSSAIN MOTTEIAN NAJAR , JOHANN HINRICH PETERS
From the Department of Immunology, University of Gouingen, West Germany
Abstract:   (4150 Views)
Human peripheral blood monocytes (HPBM) were cultured in the absence of human serum and were converted into a state exhibiting a high accessory function expressed by their ability of supporting lymphocyte proliferation. After a prolonged culture in serum free media the monocyte derived cells were highly viable, increased in size and developed veils and dendritiform elongatio'l1s. Paralleling the increase in accessory function, the cells decreased in the expression of markers typical of monocytes and macrophages (M 0), approaching the phenotype of lymphoid dendritic cells. We here define conditions for reproducibly generating these monocyte derived dendritic cells (m-DC) in various serum free media. This study also shows that acidic conditions prevent M0 development and facilitate m-DC differentiation even under serum conditions.
Full-Text [PDF 888 kb]   (1666 Downloads)    
Type of Study: Original Research | Subject: Immunology
Add your comments about this article : Your username or Email:
CAPTCHA
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.