Volume 7, Issue 1 (5-1993)                   Med J Islam Repub Iran 1993 | Back to browse issues page

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YAZDANPARAST R. SCREENING FOR STARCH-HYDROLYSING BACTERIA. Med J Islam Repub Iran 1993; 7 (1) :35-41
URL: http://mjiri.iums.ac.ir/article-1-1457-en.html
SCREENING FOR STARCH-HYDROLYSING BACTERIA
RAZIEH YAZDANPARAST
From the Institute of Biochemistry and Biophysics. Tehran University of Medical Sciences, Tehran, IsIamic Republic of Iran.
Abstract:   (4340 Views)
Screening of3000 soil samples collected from cities of four different provinces of Iran for starch-hydrolysing bacteria revealed that the nature is enriched with Streptomyces species capable of producing amylolytic enzymes. Among the bacterial isolates, one of the high starch-degrading strains was selected for further microbiological identification and also amylolytic enzyme(s) characterization. The purified isolate, Streptomyces species strain R Y 48, produces almost four times more amylolytic enzyme(s) than Bacilllls slIbtilis (PTCC 1254) on agar plates. Based on the action pattern of its amylolytic enzymes on the boiled maize starch, the purified species possesses in the logphase an amylolytic activity which is different from the amylolytic activity of the stationary phase. The secreted amylolytic enzymes which are both endolytic type and show different activity bands on native polyacrylamide gel, act independently on starch molecules.
Keywords: Amylolytic enzymes, amylases. raw-starch. Streptomyces.
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Type of Study: Original Research: Basic Science in Medicine | Subject: Biological Sciences
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