Volume 6, Issue 4 (2-1993)                   Med J Islam Repub Iran 1993 | Back to browse issues page

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NABATCHlAN F, FARZAMI B. PURIFICATIO N OF ALPHA-AMYLASE FROM BACILLUS SUBTILlS LINE # 1024 WITH ATCC 465 . Med J Islam Repub Iran 1993; 6 (4) :281-284
URL: http://mjiri.iums.ac.ir/article-1-1473-en.html
PURIFICATIO N OF ALPHA-AMYLASE FROM BACILLUS SUBTILlS LINE # 1024 WITH ATCC 465
FARIBA NABATCHlAN , BIJAN FARZAMI
From the Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, P.O. Box '•1155-5399, Tehran, Islamic Republic of lran.
Abstract:   (4322 Views)
Alpha-amylase E.C.3.2.1.1. (1,4 glucan, 4 glucanohydrolase) can be obtained from salivary glands, pancreas and microorganisms such as Pseudomonas and Asperigllus, as well as muscles and ovarian tubes.1.2 Alpha-amylase from Bacillus Subtilis #1024(A TCC 465)* was purified with a highest degree of purity in our laboratory (31.59 U/mg). The extracellular alpha-amylase was subjected to different purification techniques such as anionic and cationic exchange chromatography and preparative electrophoresis. The final fold of purification was equivalent to 38, which was higher than the previous reported values, polyacrylamide gel electrophoresis with sodium dodecyl sulphate (PAGESDS), Laemmeli method, gave a molecular weight of 82000 Daltons.
Keywords: Alpha-amylase, Bacterial enzymes, amylase electrophoresis
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Type of Study: Original Research: Basic Science in Medicine | Subject: Biological Sciences
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