From the' Department of Microbiology and Immunology, Ahwaz University of Medical Sciences, Ahwaz,
Abstract: (3397 Views)
HBs vaccines were prepared by three different methods: 1) Heat
inactivation (Krugman, et.al 1971)2) Ammonium sulphate precipitation
followed by column chromatography (Sephadex G-200) 3) PEG-6000
(polyethylene glycol) precipitation followed by column chromatography
using Sephadex G-200.
Their efficacy was studied in guinea pigs, and the results compared with
commercial Japanese vaccine (Green Cross Corporation, Osaka, Japan).
We conclude that vaccine prepared by PEG-6000 precipitation gives better
results.