Volume 19, Issue 2 (8-2005)                   Med J Islam Repub Iran 2005 | Back to browse issues page

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NAKHJAVANI F, BONAKDAR HASHEMI F, KALANi M, KAZEMI B, NOURI K, AZADI N, et al . DETECTION OF HAEMOPHILUS INFLUENZAE TYPE B IN CEREBROSPINAL FLUID OF SUSPECTED CHILDREN WITH MENINGITIS BYPCR. Med J Islam Repub Iran 2005; 19 (2) :181-184
URL: http://mjiri.iums.ac.ir/article-1-512-en.html
DETECTION OF HAEMOPHILUS INFLUENZAE TYPE B IN CEREBROSPINAL FLUID OF SUSPECTED CHILDREN WITH MENINGITIS BYPCR
F.A. NAKHJAVANI , F. BONAKDAR HASHEMI , M.T. KALANi , B. KAZEMI , K. NOURI , N. AZADI , M. HAGHI ASHTIANI , M. ALIGHOLI , U. ERFANI , M. ABEDINI
Department of microbiology. Faculty of Medicine. Tehran University of Medical Sciences , taherikalani @razi.tums.ac.ir
Abstract:   (6543 Views)
 

ABSTRACT

 

Background: A polymerase chain reaction (PCR) assay with primers from "hpd" gene was compared with culture for the detection of Haemophilus influenzae type b (Hib) in CSF samples from children suspected of meningitis.

 

Methods: 300 cerebrospinal fluid (CSF) samples from suspected meningitis cases were studied by culture (Leventhal & Chocolate Agar) and PCR.

 

Results: The latter method could detect Hib in all 5 culture positive and in 2 of 295 culture negative cases, showing sensitivity, specificity, and an accuracy index of 100%, 99% and 99%, respectively. The PCR result was available within a day.

 

Conclusion: This study has shown that PCR is a rapid, sensitive, and specific diagnostic test for Hib from CSF samples. Futthermore, to maximize management of meningitis cases to reduce the morbidity, mortality, and complications of invasive Hib infection a combination of culture and PCR is necessary for the detection ofHib infection. The incidence ofHib meningitis among children in Children's Medical Center in Tehran based on culture and PCR results were 2.4% and 1.7% respectively. Based on culture results, Hib causes 15.6% of all cases ofbacte1ial meningitis in children.

 
Keywords: Haemophilus influenzae type b; Meningitis: PCR; culture; CSF; Iran; Incidence.
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Type of Study: Original Research | Subject: Microbiology and Anatomy
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