Medical Journal of The Islamic Republic of Iran (MJIRI)
Iran University of Medical Sciences
Thu, Nov 21, 2024
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Volume 34, Issue 1 (2-2020)
Med J Islam Repub Iran 2020 |
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Golabi S, Zaringhalam J, Manaheji H. Synovial interlukin-6 affects apoptosis induction via nuclear factor kappa-B and fractalkine pathway during adjuvant arthritis. Med J Islam Repub Iran 2020; 34 (1) :168-177
URL: http://mjiri.iums.ac.ir/article-1-5501-en.html
URL: http://mjiri.iums.ac.ir/article-1-5501-en.html
Physiology Department, School of Medicine, & Neurophysiology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran , jzaringhalam@sbmu.ac.ir
Abstract: (2097 Views)
Abstract
Background and objectives: Apoptosis disruptions play substantial roles in pathogenesis of arthritis and its symptoms. Cytokines and their intra-cellular signaling have pivotal roles in arthritis pathophysiology. The present study aimed to investigate the relation between synovial Interleukin- 6 (IL-6), Nuclear Factor Kappa- B (NF-ĸB) and Fractalkine (Fkn) in the changes of edema and apoptosis during Adjuvant-induced knee arthritis.
Methods: 240 male Wistar rats were divided into different groups. Arthritis was evoked and the knee edema changes were evaluated by Vernier caliper. Synovial IL-6 was assayed by rat standard ELISA kit. Synovial NF-ĸB, Fkn, and markers of apoptosis were measured by western blot experiment.
Results: The injection of Complete Freund's Adjuvant (CFA) caused intense knee edema reduced by implementing Anti-IL-6, Anti-Fkn and Inh-NF-ĸB. The results indicated elevated levels of apoptotic markers during the acute phase, along with an increase in IL-6, NF-ĸB and Fkn. Although IL-6, NF-ĸB and Fkn levels elevation continued during chronic phase, the apoptosis markers decreased in this phase. The findings revealed that Anti-IL-6 treatment during different phases of study can change the synovial NF-ĸB and Fkn.
Conclusion: It seems that time-dependent variations in apoptotic markers level may be involved in pathogenesis of Adjuvant-induced knee arthritis. In conclusion, synovial IL-6 through NF-ĸB- Fkn pathway can play an important role in this process.
Keywords: Apoptosis, Adjuvant, Fractalkine, IL-6, Inflammation, NF-ĸB
Background and objectives: Apoptosis disruptions play substantial roles in pathogenesis of arthritis and its symptoms. Cytokines and their intra-cellular signaling have pivotal roles in arthritis pathophysiology. The present study aimed to investigate the relation between synovial Interleukin- 6 (IL-6), Nuclear Factor Kappa- B (NF-ĸB) and Fractalkine (Fkn) in the changes of edema and apoptosis during Adjuvant-induced knee arthritis.
Methods: 240 male Wistar rats were divided into different groups. Arthritis was evoked and the knee edema changes were evaluated by Vernier caliper. Synovial IL-6 was assayed by rat standard ELISA kit. Synovial NF-ĸB, Fkn, and markers of apoptosis were measured by western blot experiment.
Results: The injection of Complete Freund's Adjuvant (CFA) caused intense knee edema reduced by implementing Anti-IL-6, Anti-Fkn and Inh-NF-ĸB. The results indicated elevated levels of apoptotic markers during the acute phase, along with an increase in IL-6, NF-ĸB and Fkn. Although IL-6, NF-ĸB and Fkn levels elevation continued during chronic phase, the apoptosis markers decreased in this phase. The findings revealed that Anti-IL-6 treatment during different phases of study can change the synovial NF-ĸB and Fkn.
Conclusion: It seems that time-dependent variations in apoptotic markers level may be involved in pathogenesis of Adjuvant-induced knee arthritis. In conclusion, synovial IL-6 through NF-ĸB- Fkn pathway can play an important role in this process.
Keywords: Apoptosis, Adjuvant, Fractalkine, IL-6, Inflammation, NF-ĸB
Type of Study: Original Research |
Subject:
Physiology
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