From the Molecular Biology Unit, Pasteur institute of Iran Tehran, l.R. Iran. , Oloomi@institute.pasteur.ac.ir
Abstract: (4447 Views)
Bacterial expression systems provide economic and logistic advantages in manufacturing
proteins for human therapeutic purposes. However, most such proteins accumulate
in insoluble biologicaIIy inactive form when overexpressed in bacterial cells. This
is the case while attempting to produce recombinant human granulocyte-macrophage
colony stimulating factor (hGM-CSF) in E. coli. In this report, different strong promoters
are used to compare the yield of expression when the protein is expressed as
insoluble and soluble forms. The results obtained indicated that the level of expression
was independent of the nature of promoter used. Moreover experimental data presented
here suggest that the fusion system which directs hGM-CSF to the periplasmic
space not only can prevent inclusion body formation but also improve the level of
expression significantly.