Medical Journal of The Islamic Republic of Iran (MJIRI)
Iran University of Medical Sciences
Thu, Dec 5, 2024
[Archive]
Volume 18, Issue 2 (9-2004)
Med J Islam Repub Iran 2004 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
FARAHNEJAD Z, RASAEE M, YADEGARI H, FROUZANDEH MOGHADAM M. PURIFICATION AND CHARACTERIZATION OF CELL WALLMANNOPRO TEINS OF CANDIDA ALB/CANS USING INTACT CELL METHOD . Med J Islam Repub Iran 2004; 18 (2) :167-172
URL: http://mjiri.iums.ac.ir/article-1-638-en.html
URL: http://mjiri.iums.ac.ir/article-1-638-en.html
Department of Clinical Biochemistry , rasaee _m@modares.ac.ir.
Abstract: (4507 Views)
Virulence of the opportunistic yeast, Candida albieans, involves the interplay of
many complex changes including the yeast-hyphae transition, which mainly involves
protein changes. Cell wall mannoproteins are found to be the main cause of adherence
of C. albieans to epithel ial cells in the first step of an infection process. In the present
study, cell wall mannoproteins of intact yeast were purified using a simple treatment of
yeast with mercaptoethanol and sodium dodecyl sulfate followed by Concanavalin A
chromatography. Both electrophoretic analysis of the column effluent and Western blot
analysis using polyclonal and monoclonal antibodies showed the presence of
mannoproteins with molecular weight in the range of30-50 kDa. Dot blot analysis of
the purified antigen with the polyclonal and monoclonal antibodies prepared in this
study showed that outer membrane mannoprotein antigens were obtained successfully
following the above simple purification strategy
Type of Study: Original Research: Basic Science in Medicine |
Subject:
Clinical Biochemistry
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
