Volume 17, Issue 4 (2-2004)                   Med J Islam Repub Iran 2004 | Back to browse issues page

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MOUSAVI HOSSEINI K, POURMOKHTAR M, DINAR VAND R, REZVAN H, JALILI M A. PREPARATION OF ENRICHED IMMUNOGLOBULIN M AND IMMUNOGLOBULIN A FROM HUMAN PLASMA . Med J Islam Repub Iran 2004; 17 (4) :315-318
URL: http://mjiri.iums.ac.ir/article-1-668-en.html
PREPARATION OF ENRICHED IMMUNOGLOBULIN M AND IMMUNOGLOBULIN A FROM HUMAN PLASMA
KAMRAN MOUSAVI HOSSEINI , MOJGAN POURMOKHTAR , RASSOUL DINAR VAND , HOURI REZVAN , MOHAMMAD ALI JALILI
From the Iranian Blood Transfusion Organization Research Center and Iranian Blood Research and Fractionation Co., Tehran
Abstract:   (4302 Views)
As IgM and IgA-enriched preparations are needed to complete the immunotherapeutic spectrum, a simple procedure is described for the preparation of IgM and IgA enriched immunoglobulins. Fraction III which was prepared by cold ethanol fractionation was treated by octanoic acid followed by ethanol precipitation and ion-exchange chromatography using Sephadex DEAE A-50 and 0.1 M tris-D.35M NaCI buffer, pH 8.1, resulting in recovery of 85 % IgM, 84% IgA and 33 % IgG. The comparison of our results with immunoglobulins' percentage in plasma indicates that IgM and IgA -enrichment was obtained by three times.
Keywords: Immunoglobulin; IgM; IgA; Enrichment; Ion exchange chromatography.
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Type of Study: Original Research | Subject: Heart
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