Medical Journal of The Islamic Republic of Iran (MJIRI)
Iran University of Medical Sciences
Thu, Nov 21, 2024
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Volume 36, Issue 1 (1-2022)
Med J Islam Repub Iran 2022 |
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Mirlohi S H, Eftekhari K, Shirzadi R, Fateh A, Masoumi M, Modaresi M. The Value of Sputum Polymerase Chain Reaction for Detection of Nontuberculous Mycobacteria in Cystic Fibrosis Patients with
Negative Nontuberculous Mycobacteria Sputum Culture. Med J Islam Repub Iran 2022; 36 (1) :61-64
URL: http://mjiri.iums.ac.ir/article-1-7205-en.html
URL: http://mjiri.iums.ac.ir/article-1-7205-en.html
Seyed Hossein Mirlohi 
, Kambiz Eftekhari , Rohola Shirzadi , Abolfazl Fateh , Morteza Masoumi , Mohammadreza Modaresi
, Kambiz Eftekhari , Rohola Shirzadi , Abolfazl Fateh , Morteza Masoumi , Mohammadreza Modaresi
Pediatric Respiratory and Sleep Medicine Research Center, Children’s Medical Center, Tehran University of Medical Sciences, Tehran, Iran , modaresipul@gmail.com
Abstract: (1240 Views)
Background: Cystic Fibrosis (CF) is a life-threatening autosomal recessive disease. The purpose of this study was to evaluate the value of Polymerase Chain Reaction (PCR) in CF patients with Nontuberculous Mycobacteria (NTM) negative sputum culture.
Methods: This is a descriptive cross-sectional study. The population included all children with CF, aged between 5 - 18 years old, with an NTM negative sputum culture. The patient's sputum samples were sent for smear and culture of NTM, RFLP PCR, and PCR sequence.
Results: In total, 57 CF patients with negative NTM sputum culture were enrolled. Nine patients (15.78%) had positive sputum PCR for NTM. Among these strains, Mycobacterium simiae was the most common one with 5 cases (8.77% of total positive cases).
Conclusion: PCR can be used as an alternative diagnostic method for NTM in CF patients with negative NTM sputum culture, always under clinical suspicion of the disease.
Methods: This is a descriptive cross-sectional study. The population included all children with CF, aged between 5 - 18 years old, with an NTM negative sputum culture. The patient's sputum samples were sent for smear and culture of NTM, RFLP PCR, and PCR sequence.
Results: In total, 57 CF patients with negative NTM sputum culture were enrolled. Nine patients (15.78%) had positive sputum PCR for NTM. Among these strains, Mycobacterium simiae was the most common one with 5 cases (8.77% of total positive cases).
Conclusion: PCR can be used as an alternative diagnostic method for NTM in CF patients with negative NTM sputum culture, always under clinical suspicion of the disease.
Type of Study: Original Research: Clinical Science |
Subject:
Pulmonary Disease
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