Volume 15, Issue 3 (11-2001)                   Med J Islam Repub Iran 2001 | Back to browse issues page

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BAMDAD T, RODSTAI M, SOLIMANDJAHI H, MALEKANEH M. APPLICATION AND ADAPTATION OF DOT IMMUNOASSAY TO DETECT AND MEASURE RUBELLA VIRUS ANTIBODY. Med J Islam Repub Iran 2001; 15 (3) :171-174
URL: http://mjiri.iums.ac.ir/article-1-801-en.html
APPLICATION AND ADAPTATION OF DOT IMMUNOASSAY TO DETECT AND MEASURE RUBELLA VIRUS ANTIBODY
T BAMDAD , MH RODSTAI , H SOLIMANDJAHI , M MALEKANEH
From the Department of Virology. Facility a/Medical Sciences. Tarbiat Modarres University. Po. Box 14115-111. Tehran
Abstract:   (3869 Views)
A dot immunobinding assay ( DIA) was used for a quantitative and qualitative assay of rubella antibody. Purified antigen and conjugated anti-human immunoglobulin (RAHlg) were prepared. Nitrocellulose paper dotted with the antigen was added to serially diluted sera or blood samples. The reacting antibodies were visualized by a peroxidase system. Development of a colored insoluble substrate was taken as a positive result. The adapted DIA was applied to test 105 serum samples. The sensitivity and specificity and immune titer of DIA were compared with hemagglutination inhibition (HI) test.
Keywords: Rubella virus. dot immunoassay ( DIA), nitrocellulose paper. hemagglutination inhibition (HI)
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Type of Study: Original Research: Basic Science in Medicine | Subject: Biological Sciences
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