Volume 15, Issue 2 (8-2001)                   Med J Islam Repub Iran 2001 | Back to browse issues page

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NASIRI S, REZVAN H, MOUSAVI K, ROOSTAEI M. PREPARATION OF HIGHLY PURIFIED SOLVENTDETERGENT COA GULATION FACTOR VII AND FACTOR IX CONCENTRATES FROM PROTHROMBIN COMPLEX (PPSB). Med J Islam Repub Iran 2001; 15 (2) :103-108
URL: http://mjiri.iums.ac.ir/article-1-812-en.html
PREPARATION OF HIGHLY PURIFIED SOLVENTDETERGENT COA GULATION FACTOR VII AND FACTOR IX CONCENTRATES FROM PROTHROMBIN COMPLEX (PPSB)
S NASIRI , H REZVAN , K MOUSAVI , MH ROOSTAEI
From the Department of Research and Development, Blood Research and Fractionation Co., Tehran, and the Department of Virology, Tarbiat Modarres University, Tehran, I.R. Iran.
Abstract:   (4674 Views)
In this study, anion-exchange chromatography was used to purify factor VII and factor IX from prothrombin complex (PPSB), which contains coagulation factors II, VII, IX and X. For this purpose, DEAE-Sepharose CL-6B gel , Pharmacia column XK-26 , high flow rate and two stepwise gradients with phosphate citrate buffer were used. The yield of the two lyophylized products, factor VII and factor IX concentrates, was 61 % and 75%, respectively. Specific activity of factor VII increased from 0.16 to 3.06 (purification-fold= 19.1) and specific activity of factor IX increased from 12 to 4.9 (purification-fold= 4.1). Results of electrophoresis on agarose gel-as well as immunoelectrophoresis-indicated higher purity of factor VII and factor IX compared to PPSB. Thrombogenicity of the two products were within the normal range defined for PPSB. In order to improve viral safety, solvent-detergent treatment was performed prior to further purification. Factor VII concentrate is used for patients with factor VII deficiency and also for hemophilia patients with inhibitors. Factor IX concentrate is used for treatment of hemophilia B patients.
Keywords: Factor VII, Factor VIla, Factor IX, Hemophilia, PPSB, Viral inactivation.
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Type of Study: Original Research: Basic Science in Medicine | Subject: Heart
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