Volume 15, Issue 1 (5-2001)                   Med J Islam Repub Iran 2001 | Back to browse issues page

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FOTOHI F, ROUSTAEE M, SOLEIMANGAHI H, KHABIRI A, MALEKANE M, SABAHI F. APPLICATION OF DOT IMMUNOASSAY (DIA) FOR DETECTION AND TITRATION OF MEASLES VIRUS ANTIBODIES. Med J Islam Repub Iran 2001; 15 (1) :31-35
URL: http://mjiri.iums.ac.ir/article-1-822-en.html
APPLICATION OF DOT IMMUNOASSAY (DIA) FOR DETECTION AND TITRATION OF MEASLES VIRUS ANTIBODIES
F FOTOHI , MH ROUSTAEE , H SOLEIMANGAHI , AR KHABIRI , M MALEKANE , F SABAHI
From the Department of Virology, School of Medical Sciences, Tarbiat Modarres University, Tehran, Islamic Republic of Iran
Abstract:   (4873 Views)
Measles is one of the most contagious human diseases. Although mass vaccination programs have reduced the incidence of this disease, measles is still an important cause of morbidity and mortality among children in developing countries. Therefore the use of sensitive techniques to evaluate vaccine efficacy and level of immunity among members of susceptible communities is crucial. Serum neutralization test (SNT) and Dot immunoassay (DIA) are among the best methods utilized for evaluating measles virus antibodies. In this study, DIA was applied for detection and titration of measles virus antibodies. This test was developed for the first time in Iran in the Virology Department of the School of Medical Sciences, Tarbiat Modarres University. Viral antigen was first prepared and titrated. Then human IgG was isolated by affinity chromatography. Anti-human immunoglobulin was prepared by immunizing rabbits with human IgG and was later conjugated with peroxidase. DIA was applied using these reagents. The results indicated that the specificity and sensitivity of DIA in comparison with SNT was 96% and 89%, respectively. This study demonstrated that DIA is a rapid and simple test which can be applied for the detection of mass immunity against the measles virus.
Keywords: Measles virus, Serum neutralization test, DIA, Anti-human immunoglobulin.
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Type of Study: Original Research | Subject: General
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