Volume 15, Issue 1 (5-2001)                   Med J Islam Repub Iran 2001 | Back to browse issues page

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ZAMANI Z, ASSMAR M, PYAZAK N, WAFAIE K, ASSADIAN M, AMIRKHANI A. A NOVEL METHOD OF PURIFICATION OF SPECIFIC HYDATID CYST ANTIGEN. Med J Islam Repub Iran 2001; 15 (1) :49-53
URL: http://mjiri.iums.ac.ir/article-1-825-en.html
A NOVEL METHOD OF PURIFICATION OF SPECIFIC HYDATID CYST ANTIGEN
Z ZAMANI , M ASSMAR , N PYAZAK , K WAFAIE , M ASSADIAN , A AMIRKHANI
From the Departments of Parasitology and Biochemistry, Pasteur Institute of Iran, Tehran, I.R. Iran.
Abstract:   (4603 Views)
Hydatid cyst fluid has been used as a source of antigen for the serodiagnosis of Echinococcus granulosus infection. Due to cross-reactions with antigens shared by other helminthes, the specific antigen from hydatid cyst fluid was purified by many workers. We used a relatively simple technique for purification of specific antigen from sheep hydatid cyst fluid. Isopycnic ultracentrifugation with 30% KEr was used followed by SDS-PAGE to check the purity of the antigen. The antigen was of 48 kDa and used in ELISA and IRA with high sensitivity and specificity.
Keywords: Echinococcus granulosus, specific antigen, isopycnic ultracentrifugation
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Type of Study: Original Research: Basic Science in Medicine | Subject: Parasitology and Mycology
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