From the Division of Immunology. Faculty of Medicine. Tabriz University of Medical Sciences. Tabriz. I. R. Iran
Abstract: (4664 Views)
In vitro culture systems using bone marrow cells from BALB/C mice were set up
in medium supplemented with spleen-derived medium. Bone marrow cells grown in
spleen-derived medium gave rise to cultures containing >97% mast cells. The cells
were used in polarisation chemotaxis assays with the intention of determining the
effect of nerve tissue-derived neurotransmitters on mast cell migration. Some available
neurotransmitters including (substance-p, histamine, serotonin, Dopa, noradrenaline,
acetylcholine, aspartic acid, epinephrine and nerve extracts) were tested. Mast
cells showed a significant morphological response to 10-3 and 10-4 M histamine, 10-9
M serotonin, and 10'2 M Dopa Nerve extracts induced some shape changes in mast cells.