Volume 14, Issue 3 (11-2000)                   Med J Islam Repub Iran 2000 | Back to browse issues page

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QUJEQ D, USEFNIA PASHA Q. DEVELOPMENT OF A METHOD FOR THE DETERMINATION OF 4-HYDROXY-3-METHOXYMANDELIC ACID (VANILLYLMANDELIC ACID) IN URINE BY PAPER CHROMATOGRAPHY. Med J Islam Repub Iran 2000; 14 (3) :267-270
URL: http://mjiri.iums.ac.ir/article-1-863-en.html
DEVELOPMENT OF A METHOD FOR THE DETERMINATION OF 4-HYDROXY-3-METHOXYMANDELIC ACID (VANILLYLMANDELIC ACID) IN URINE BY PAPER CHROMATOGRAPHY
DURDI QUJEQ , Q USEFNIA PASHA
From the Department of Biochemistry and Biophysics, Babol University of Medical Sciences, Babol, I. R. Iran.
Abstract:   (4063 Views)
We have developed a simple and precise paper chromatographic method for the determination of 4-hydroxy-3-methoxymandelic acid (VMA) in urine. Concentrations of VMA in patients with neuroblastoma were increased in comparison to controls. The linearity was excellent in the concentration range tested. The within-assay coefficient of variation for control and patient urine was less than 2.2%. The recovery was in the range of 97.9-99.4%. Results from testing urine samples of controls and patients with neuroblastoma suggest that this method is a reliable and convenient system for quantification of VMA in urine and can be used in the mass screening of neuroblastoma in infants. Sample preparation requires minimal time and the entire procedure is completed within 5 h.
Keywords: Vanillylmandelic acid, paper chromatography, neuroblastoma.
Full-Text [PDF 151 kb]   (1723 Downloads)    
Type of Study: Original Research | Subject: Biological Sciences
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