Volume 13, Issue 3 (11-1999)                   Med J Islam Repub Iran 1999 | Back to browse issues page

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TAJERZADEH H, SADRAY S. HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF THEOPHYLLINE IN HUMAN SERUM. Med J Islam Repub Iran 1999; 13 (3)
URL: http://mjiri.iums.ac.ir/article-1-936-en.html
HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF THEOPHYLLINE IN HUMAN SERUM
H TAJERZADEH , S SADRAY
From the Biopharmacy Division, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IR.Iran, 14155/6451.
Abstract:   (4286 Views)
A simple, rapid and sensitive HPLC method for the determination of theophylline (T) in human serum has been developed. An isocratic system consisting of a /-l Bondapak C18 column, mobile phase of methanol, phosphate buffer (22:78, pH=4.5), and a flow rate of 1.4 mL/min was used. The eluent was detected by UV at 275 nm at room temperature. 8-Chlorotheophylline (8-CT) was used as an internal standard. The serum samples deproteinated by methanol containing 8-CT and the supernatant was injected into the HPLC system. The retention times of 5.7 and 8.1 min were found for T and 8-CT, respectively. The linearity was checked in the range of 0.2- 30 µg/mL. Relative standard deviation for both inter-day and intra-day precision analysis was less than 5%. No interference was observed from endogenous serum components. Specificity was shown against some commonly co-administered drugs. Simple and fast sample preparation, small sample volume (250 µL), precision, reproducibility, specificity, sensitivity and high percentage recovery (98 %) make the method to be practically useful for T monitoring in asthmatic patients.
Keywords: Theophylline; Reversed-phasechromatography; 8-Chlorotheophylline; Therapeutic drug monitoring.
Full-Text [PDF 410 kb]   (3569 Downloads)    
Type of Study: Original Research | Subject: Pharmacology
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