Volume 13, Issue 3 (11-1999)                   Med J Islam Repub Iran 1999 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
ESMAILNEJAD MOGHADDAM A, KARIMPOUR MALEKSHAH A, HIROI M, SAITO H. INTRACYTOPLASMIC SPERM INJECTION (lCSI) IN MICE BY A NEW HOLDING PIPETTE. Med J Islam Repub Iran 1999; 13 (3) :219-223
URL: http://mjiri.iums.ac.ir/article-1-940-en.html
INTRACYTOPLASMIC SPERM INJECTION (lCSI) IN MICE BY A NEW HOLDING PIPETTE
AMIR ESMAILNEJAD MOGHADDAM , ABBASALI KARIMPOUR MALEKSHAH , MASAHIKO HIROI , HIDEKAZU SAITO
From the Department of Anatomy and Embryology, Medical School, Mazandaran University of Medical Sciences, Sari, I R. Iran
Abstract:   (15208 Views)
Mice are ideal models for ICSI experiments because of the ease of culturing eggs/embryos in vitro and the availability of ample genetic information. Unfortunately, it has been extremely difficult. In this study we improved the mouse ICSI method by using a new holding pipette that was made of two pipettes such that one pipette was pulled and heat merged into the other one. The outer pipette had an outer diameter of 120 J..l. and an inner diameter of 80 J..l. to 85 J.1 (about mouse oocyte diameter). The inner pipette had an outer diameter of 80 µ to 85 µ. and an inner diameter of 55 µ to 60 µ. which was polished and narrowed to 20 J..l.on amicroforge. The distance between the tips of the two pipettes was adjusted to 120 µ. to 160 µ (1.5 to 2 fold longer than mouse oocyte diameter). Of 307 oocytes which were injected with a single spermatozoon, 206 (67.1 %) survived and 93 (45.1 %) of surviving oocytes showed normal fertilization (2 pronuclei and second polar body). Of 109 oocytes which were only sucked into the holding pipette (control group), 105 (96.3%) survived and only 4 (3.8%) of them became activated parthenogenically. U sing this new holding pipette, the oocyte is sucked into a glass tunnel and elongates to a reasonable length therefore the injection axis will be increased and piercing of the oolemma can be performed easily.
Keywords: Intracytoplasmic spenn injection, mouse ICSI, holding pipette
Full-Text [PDF 646 kb]   (1435 Downloads)    
Type of Study: Original Research: Basic Science in Medicine | Subject: Anatomical Sciences
Add your comments about this article : Your username or Email:
CAPTCHA
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.