OLOOMI M, BOUZARI S, RECHINSKY V O. PURIFICATION AND CHARACTERIZATION OF THE CLONED HUMAN GM-CSF GENE EXPRESSED IN ESCHERICHIA COLI. Med J Islam Repub Iran 1999; 12 (4) :353-357
URL:
http://mjiri.iums.ac.ir/article-1-987-en.html
From the Molecular Biology Unit, Pasteur Institute of Iran, Tehran I.R. Iran.
Abstract: (4705 Views)
The human granulocyte-macrophage colony stimulation factor (hGM-CSF)
gene was cloned in the pET 23a( +) expression vector under the control of strong
bacteriophage T7 transcription and translation signals. The hGM-CSF gene was
transferred into E. coli strainBL21 (DE3)pLysS andIPTG was used for induction
of GM-CSF gene. Production of the target protein was obtained as revealed by
ELISA and Western blot analysis. The produced 'h:GM-CSF was purified by
immunoaffinity chromatography. The dot blot positive fractions were assayed for
biological activity and it was shown that the expressed GM-CSF is active.