Volume 10, Issue 4 (2-1997)                   Med J Islam Repub Iran 1997 | Back to browse issues page

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ZIA FAZELI T, RABBANI A. EFFECTS OF SERUM AND PROTEIN SYNTHESIS INHIBITORS ON POLYMORPHONUCLEAR NEUTROPHIL APOPTOSIS. Med J Islam Repub Iran 1997; 10 (4) :285-289
URL: http://mjiri.iums.ac.ir/article-1-1155-en.html
EFFECTS OF SERUM AND PROTEIN SYNTHESIS INHIBITORS ON POLYMORPHONUCLEAR NEUTROPHIL APOPTOSIS
TAHEREH ZIA FAZELI , AZRA RABBANI
From the Institute of Biochemistry and Biophysics, University of Tehran, P.O. Box 13145-1384, Tehran, Islamic Republic of Iran.
Abstract:   (4010 Views)
In this study the programmed cell death (apoptosis) of human neutrophilic granulocytes was investigated in the presence and absence of fetal calf serum (FCS), cycloheximide and actinomycin-D. The results show that when FCS is omitted from cultures, apart from a decrease in viability, the percentage of apoptotic cells and DNA fragmentation increases. Apoptosis is accelerated in serum withdrawal cultures at 6 hours of incubation time. The use of fluorescent dyes and diphenylamine reaction proceaures confirm the above results. Treatment of cells with protein synthesis inhibitors, actinomycin-D and cycloheximide promotes apoptosis and produces a typical ladder of internucleosomal cleavage in the cellular chromatin the extent of fragmentation however, differs.
Keywords: Apoptosis, polymorphonuclear neutrophil, cycloheximide, actinomycin D.
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Type of Study: Original Research: Basic Science in Medicine | Subject: Biological Sciences
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