BAMDAD T, RODSTAI M, SOLIMANDJAHI H, MALEKANEH M. APPLICATION AND ADAPTATION OF DOT IMMUNOASSAY TO DETECT AND MEASURE RUBELLA VIRUS ANTIBODY. Med J Islam Repub Iran 2001; 15 (3) :171-174
URL:
http://mjiri.iums.ac.ir/article-1-801-en.html
From the Department of Virology. Facility a/Medical Sciences. Tarbiat Modarres University. Po. Box 14115-111. Tehran
Abstract: (3926 Views)
A dot immunobinding assay ( DIA) was used for a quantitative and qualitative
assay of rubella antibody. Purified antigen and conjugated anti-human immunoglobulin
(RAHlg) were prepared. Nitrocellulose paper dotted with the antigen
was added to serially diluted sera or blood samples. The reacting antibodies were
visualized by a peroxidase system. Development of a colored insoluble substrate
was taken as a positive result. The adapted DIA was applied to test 105 serum
samples. The sensitivity and specificity and immune titer of DIA were compared
with hemagglutination inhibition (HI) test.