Volume 13, Issue 1 (5-1999)                   Med J Islam Repub Iran 1999 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
AHMADI H, TABARAlE B, NEJATI M, AL-e AGHA S. MOLECULAR CHARACTERIZATION AND OPTIMIZATION OF VI-CAPSULAR POLYSACCHARIDE OF SALMONELLA TYPHI TY6S PRODUCTION IN BIOREACTOR. Med J Islam Repub Iran 1999; 13 (1) :37-42
URL: http://mjiri.iums.ac.ir/article-1-970-en.html
MOLECULAR CHARACTERIZATION AND OPTIMIZATION OF VI-CAPSULAR POLYSACCHARIDE OF SALMONELLA TYPHI TY6S PRODUCTION IN BIOREACTOR
HOJJAT AHMADI , BAHMAN TABARAlE , MEHDI NEJATI , SAEID AL-e AGHA
From the Department of Bacterial Vaccine. Research and Production Complex, Pasteur Institute of Iran,
Abstract:   (4836 Views)
The role of Vi-capsular polysaccharide (Vi-CPS) in human immunity against infection caused by Salmonella typhi is well known. The downstream process of purification generally causes depolymerization of Vi-CPS to a nonimmunogenic low molecular weight form. In the present study, a standard strain of Sal. typhi Ty6s was grown under submerge cultural conditions in a pilot-plant scale of 90 Liter fermentor. At the late exponential growth phase, crude polysaccharide wa obtained from fermentation broth by detergent-phenol extraction method and purified by ultracentrifuge differentiation technique. Analytical data reveals that by optimization of fermentation parameters, not only was the yield of production increased from 1.6 mg/L to 4.9 mg/L, but also the polysaccharide retained its native molecular stability and immunogenicity. Therefore, purified Vi-CPS can be regarded as a reliable immunogen to control typhoid fever in man.
Keywords: Salmonella typhi. Vi-CPS, Polysaccharide vaccine.
Full-Text [PDF 2317 kb]   (2904 Downloads)    
Type of Study: Original Research: Basic Science in Medicine | Subject: Artificial intelligence
Add your comments about this article : Your username or Email:
CAPTCHA
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.