Volume 12, Issue 4 (2-1999)                   Med J Islam Repub Iran 1999 | Back to browse issues page

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OLOOMI M, BOUZARI S, RECHINSKY V O. PURIFICATION AND CHARACTERIZATION OF THE CLONED HUMAN GM-CSF GENE EXPRESSED IN ESCHERICHIA COLI. Med J Islam Repub Iran 1999; 12 (4) :353-357
URL: http://mjiri.iums.ac.ir/article-1-987-en.html
PURIFICATION AND CHARACTERIZATION OF THE CLONED HUMAN GM-CSF GENE EXPRESSED IN ESCHERICHIA COLI
MANA OLOOMI , SAEID BOUZARI , VLADIMIR O RECHINSKY
From the Molecular Biology Unit, Pasteur Institute of Iran, Tehran I.R. Iran.
Abstract:   (4707 Views)
The human granulocyte-macrophage colony stimulation factor (hGM-CSF) gene was cloned in the pET 23a( +) expression vector under the control of strong bacteriophage T7 transcription and translation signals. The hGM-CSF gene was transferred into E. coli strainBL21 (DE3)pLysS andIPTG was used for induction of GM-CSF gene. Production of the target protein was obtained as revealed by ELISA and Western blot analysis. The produced 'h:GM-CSF was purified by immunoaffinity chromatography. The dot blot positive fractions were assayed for biological activity and it was shown that the expressed GM-CSF is active.
Keywords: Human GM-CSF, Gene Expression, Purification, E. coli.
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Type of Study: Original Research: Basic Science in Medicine | Subject: Biological Sciences
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