Volume 2, Issue 3 (11-1988)                   Med J Islam Repub Iran 1988 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
A. GHAVAMI NEJAD A, ZANDIEH T. INHIBITION OF14C UPTAKE BY CANDIDA ALBICANS: A NEW METHOD FOR INVESTIGATION OF INTRACELLULAR KILLING BY PHAGOCYTES. Med J Islam Repub Iran 1988; 2 (3) :237-242
URL: http://mjiri.iums.ac.ir/article-1-1255-en.html
INHIBITION OF14C UPTAKE BY CANDIDA ALBICANS: A NEW METHOD FOR INVESTIGATION OF INTRACELLULAR KILLING BY PHAGOCYTES
A A. GHAVAMI NEJAD , T ZANDIEH
From the Department of Biology, Faculty of Sciences, Isfahan University of Medical Sciences, Isfahan,
Abstract:   (3966 Views)
A radio assay employing 14C-uridine to measure the intracellular killing of Candida albicans by phagocytes is presented. The principle of this new method is that after killing of Candida albicans by phagocytes RNA synthesis stops and therefore uridine uptake is diminished whilst viable candida in cultures would take up uridine and as a result, high CPM counts can be obtained within 135 min. of incubation in test cultures. Leukocytes from 30 normal subjects showed reduced C.P.M. following killing of Candida albicans.
Full-Text [PDF 560 kb]   (1368 Downloads)    
Type of Study: Original Research: Basic Science in Medicine | Subject: Biological Sciences
Add your comments about this article : Your username or Email:
CAPTCHA
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.